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Human sperm chromosomes were obtained after capacitation with TES‐Tris (TEST) yolk buffer and fusion with Syrian hamster eggs. Semen samples could be stored at 4°C for 3 days and remain functional in the assay system. The efficiency of TEST yoik buffer for obtaining karyotypes was as good as, or greater than, the efficiency of standard BWW medium containing human serum albumin.
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Brandriff et al. (1985) studied this question.
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