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This research examines the efficiency of DNA ligation and recombination in vertebrate cells during transfection.

May 6, 1983Science

High-Efficiency Ligation and Recombination of DNA Fragments by Vertebrate Cells

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Randomized trial analyzes ligation efficiency of DNA fragments in vertebrate cells, suggesting high success rates in transfection.

This research examines the efficiency of DNA ligation and recombination in vertebrate cells during transfection.
Quantitatively analyzed DNA infectivity from avian retrovirus and DNA fragment mixtures.
Evaluated uptake of two DNA molecules in a single cell.
Assessed efficiency of ligation and recombination of unlinked DNAs.
Transfected cells showed nearly 100% efficiency in ligation and recombination of DNA fragments.
Successful uptake of both DNA molecules occurred in a biologically active state.

Miller et al. (1983) studied this question.

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