What question did this study set out to answer?

The aim is to isolate the human genes for insulin-like growth factors to understand their genomic arrangement.

Isolation of the human insulin-like growth factor genes: insulin-like growth factor II and insulin genes are contiguous.

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Isolates human IGF-I and IGF-II genes to demonstrate their adjacent existence, suggesting gene regulation insights.

The aim is to isolate the human genes for insulin-like growth factors to understand their genomic arrangement.
Isolated overlapping recombinant clones from a human genomic DNA library.
Analyzed exon-intron organization and restriction endonuclease cleavage maps of IGF-II and insulin genes.
Investigated gene adjacency using hybridization with an IGF-II cDNA probe.
IGF-I gene spans over 35 kbp and IGF-II gene spans at least 15 kbp in the human genome.
IGF-II and insulin genes are adjacent with 12.6 kbp of intergenic DNA containing Alu sequences.
Both genes follow the same polarity with four exons, differing from the insulin gene.

Bell et al. (1985) studied this question.

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