Key result
NPRA protein was highly expressed in 71.1% of esophageal squamous cell carcinoma tissues compared to 17.5% of non-tumor tissues, and its expression correlated with TNM stage and histologic differentiation.
Why the study?
Does NPRA knockdown reduce cell migration and invasion in esophageal squamous cell carcinoma cells?
Observational (n=45)
No
Does NPRA knockdown reduce cell migration and invasion in esophageal squamous cell carcinoma cells?
Absolute Event Rate: 71.1% vs 17.5%
p-value: p=0.000
NPRA is highly expressed in esophageal squamous cell carcinoma and promotes tumor cell migration and invasion in vitro, suggesting it may be a potential therapeutic target.
NPRA overexpression may mark aggressive esophageal squamous cell carcinoma; leaves open its value as a therapeutic target.
The natriuretic peptide receptor-A (NPRA) has been investigated as a receptor of natriuretic peptides in the cardiovascular system. In this study, however, we analyze the expression status of NPRA and the relationship with tumor invasion in esophageal squamous cell carcinoma (ESCC) for the first time. Western blots were used to examine the expression status of protein in human ESCC cell lines. Then, we used immunohistochemistry to detect the expression of NPRA in 45 ESCC specimens and 40 corresponding nontumor tissues. The clinical data were analyzed through statistical methods. Sh-RNA-NPRA was transfected into Eca109 cells to detect the relationship between NPRA and cell invasion through transwell assays. In esophageal squamous cells, the expression of NPRA was strongly detected in the cytoplasm, while undetectable or very weak in the nucleus. The positive rates of NPRA in cancer tissues are significantly higher than that in nontumor tissues (P <0.05). Clinicopathological analyses revealed that increased NPRA expression correlated with differentiation and TNM stage (P <0.05), while it showed no statistically significant association with age, gender, and lymph node metastasis. In analysis of prognosis, we found that highly.Transwell assays showed that NPRA promoted Eca109 cell migration and invasion in vitro and may be involved in MMP2 and MMP9 activation. NPRA protein is highly expressed in ESCC tissues and could promote Eca109 cell migration and invasion in vitro.
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Zhao et al. (2014) conducted an observational in Esophageal squamous cell carcinoma (ESCC) (n=45). NPRA expression vs. Nontumor tissues was evaluated on High NPRA protein expression (p=0.000). NPRA protein was highly expressed in 71.1% of esophageal squamous cell carcinoma tissues compared to 17.5% of non-tumor tissues, and its expression correlated with TNM stage and histologic differentiation.
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