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SUMMARY: Serum-free chemically defined media were developed for cultivating several Leptospira strains. Leptospira canicola, Ruebeusch strain, was the main test strain. Esterified fatty acids (monoolein, monostearin, methyl palmitate, methyl oleate) replaced serum. Fe++ over a narrow range replaced haemin. Thiamine was essential. Other vitamins added to thiamine-containing medium (nicotinic acid, pantothenate, putrescine, p-hydroxybenzoic acid, vitamin B12) speeded or increased final growth. Acetate stimulated growth moderately. Ammonium sulphate was favourable over a wide concentration range.
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Vogel et al. (1961) studied this question.
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