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The response of Ochromonas malhamensis, Euglena gracilis, and Lactobacillus leichmannii to vit. B12 in normal urine and in urine of patients, given an intramuscular dose of 50 μg of vit. B12 was studied. The “true” vit. B12 values presumably are given by the Ochromonas malhamensis assay. The response of E. gracilis and L, leichmannii to other members of the B12 group and to other metabolites is discussed from the standpoint of how to estimate responses other than O. malhamensis-active B12. Calculations are given for the estimation of interfering metabolites.
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Baker et al. (1956) studied this question.
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