Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells
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Discussion
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Implication
Randomized trial isolates a cDNA encoding CD34 from hematopoietic cells, suggesting its role in stem cell biology.
Key Points
- This research aims to clone and characterize the CD34 gene, a sialomucin associated with hematopoietic stem cells.
- Isolated CD34 cDNA from a KG-1 cell library using transient expression in COS cells and anti-CD34 antibodies.
- Amplified 5' and 3' ends of the full-length cDNA using PCR; the final clone had 2615 bp and a poly(A) tail.
- Analyzed CD34 expression in various hematopoietic and endothelial cell lines through Southern blot and immunoprecipitation techniques.
- The cDNA clone expressed a surface protein of approximately 110 kDa in transfected COS cells.
- A 2.7-kb CD34 transcript was identified in specific hematopoietic cell lines but not in others, indicating selective expression.
- CD34 exhibits unique glycosylation sites and phosphorylation sites, hinting at its function in cellular signaling.
Cite This Study
Simmons et al. (1992) studied this question.