Pacing-induced heart failure in rabbits impairs the functions of L-type Ca2+ channels, Na+/Ca2+ exchanger, and SR Ca2+-ATPase, resulting in reduced [Ca2+]i transients and ventricular dysfunction.
To determine whether there are abnormalities in myocyte excitation-contraction coupling and intracellular Ca2+ concentration (Ca2+i) homeostasis in pacing-induced heart failure (PF), we measured L-type Ca2+ current (ICa,L) and Na+/Ca2+ exchanger current (INa/Ca) with voltage clamp and measured intracellular Na+ concentration (Na+i) and Ca2+i with the use of sodium-binding benzofuran isophthalate (SBFI) and fluo 3 in ventricular myocytes isolated from control and paced rabbits. The peak systolic and diastolic levels and the amplitude of electrically stimulated Ca2+i transients (0.25 Hz, extracellular Ca2+ concentration = 1.08 mM) were significantly less in PF myocytes. Also, there was prolongation of the times to peak and decline of Ca2+i transients. ICa,L density was markedly decreased in PF myocytes. INa/Ca at -40 mV elicited by rapid exposure to 0 Na+ solution with a rapid solution switcher was significantly reduced in PF myocytes, suggesting that the function of the Na+/Ca2+ exchanger is impaired in these myocytes. In PF myocytes the decline of the Ca2+i transient when the Na+/Ca2+ exchanger was abruptly disabled was markedly prolonged compared with the decline in control myocytes, consistent with depressed sarcoplasmic reticulum (SR) Ca2+-ATPase function. RNase protection assay showed decreased levels of Na+/Ca2+ exchanger and SR Ca2+-ATPase mRNA in PF hearts, consistent with the function studies. We conclude that the functions of L-type Ca2+ channels, Na+/Ca2+ exchanger, and SR Ca2+-ATPase are impaired in myocytes from rabbit hearts with failure induced by rapid pacing. These abnormalities result in reduced Ca2+i transients and systolic and diastolic dysfunction and appear to account for the abnormal ventricular function observed.
Yao et al. (Thu,) conducted a other in Pacing-induced heart failure. Pacing-induced heart failure vs. Control rabbits was evaluated on L-type Ca2+ current, Na+/Ca2+ exchanger current, and intracellular Ca2+ concentration transients. Pacing-induced heart failure in rabbits impairs the functions of L-type Ca2+ channels, Na+/Ca2+ exchanger, and SR Ca2+-ATPase, resulting in reduced [Ca2+]i transients and ventricular dysfunction.