A refined plating medium designed to enumerate E. coli in food within 24 hr was developed. The medium combined tergitol 7 with the fluorogenic substrate, 4‐methylumbelliferyl β‐D‐glucuronide. E. coli colonies were detected by fluorescence under long‐wave UV light. The new agar allowed superior recovery of heat and freeze‐stressed cells and produced E. coli counts from meat and poultry samples at levels generally equivalent to or better than those of the standard 5 tube Most Probable Number method. This method provides a simple and rapid alternative to traditional techniques.
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DAMARÉ et al. (1985) studied this question.
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