Development and validation of an LC-MS/MS method for the quantification of novel therapeutic TT-478, a selective adenosine receptor 2B antagonist, for a phase I/II clinical trial

BACKGROUND: TT-478 is a novel adenosine receptor 2B antagonist, administered orally as a prodrug (TT-702) for the treatment of advanced metastatic prostate cancer in a Phase I/II clinical trial setting. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was required to quantify TT-478 in plasma samples obtained from patients recruited to the ongoing early-phase trial. METHODS AND RESULTS: An LC-MS/MS method has been developed and fully validated that allows the quantification of TT-478 in patient plasma samples following a simple extraction procedure using acetonitrile. The assay was shown to be sensitive and selective for TT-478, with an analytical range of 75-25, 000 ng/mL, and exhibited excellent precision (coefficient of variation < 12%) and accuracy in the range of 96-107%. Consistently high recovery was achieved, and no matrix effect observed. Analysis of patient samples confirmed that TT-702 rapidly and completely hydrolyzes to TT-478 following administration. CONCLUSION: https: //clinicaltrials. gov/study/NCT05272709? term=AREA%5BConditionSearch%5D (Advanced%20Prostate%20Cancer) %20AND%20AREA%5BBasicSearch%5D (phase%203%20drug) %20AND%20AREA%5BOverallStatus%5D (NOTYETRECRUITING%20OR%20RECRUITING%20OR%20ENROLLINGBYINVITATION%20OR%20ACTIVENOTRECRUITING) &rank=10 identifier is NCT05272709.