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November 1, 1990Journal of VirologyOpen Access

A region of the 5' noncoding region of foot-and-mouth disease virus RNA directs efficient internal initiation of protein synthesis within cells: involvement with the role of L protease in translational control

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Population

Cells transfected with plasmids encoding bicistronic mRNAs

Comparison

435-nt region from the 5' noncoding region of… vs Deletion mutants

Design

Preclinical

Authors

GBGraham J. BelshamStatens Serum InstitutJBJ K BrangwynThe Pirbright Institute

Discussion

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Overview

Defines minimal FMDV IRES boundaries; leaves open translation to pathogenesis models and antiviral strategies.

Structured PICO

P
Population
Cells transfected with plasmids encoding bicistronic mRNAs
I
Intervention
435-nt region from the 5' noncoding region of foot-and-mouth disease virus (FMDV)
C
Comparator
Deletion mutants (loss of ~30 nt from 5' terminus or ~50 nt from 3' terminus)
O
Outcome
Internal initiation of protein synthesis

Identified a specific 435-nt region in the FMDV 5' noncoding region essential for internal initiation of protein synthesis and demonstrated that FMDV L protease inhibits expression of genes lacking this element.

Cite This Study

Belsham et al. (1990) studied this question.

synapsesocial.com/papers/6a225cce7d05348b2ab600d2https://doi.org/10.1128/jvi.64.11.5389-5395.1990
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo1989 · 419 citations
  2. 2Potential secondary and tertiary structure in the genomic RNA of foot and mouth disease virus1987 · 100 citations
  3. 3Relationship of p220 cleavage during picornavirus infection to 2A proteinase sequencing1988 · 144 citations
  4. 4Leader protein of foot-and-mouth disease virus is required for cleavage of the p220 component of the cap-binding protein complex1988 · 320 citations
  5. 5Use of modified T7 DNA polymerase (sequenase version 2.0™) for oligonucleotide site-directed mutagenesis1989 · 14 citations