Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo
Population
COS-1 cells transfected with expression vectors yielding mono-, di-, and tricistronic mRNAs
Comparison
5' nontranslated region of encephalomyocarditis… vs Deletion in the EMCV 5'NTR, or capped globin 5'NTR
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Highlights potential IRES targets for antivirals; leaves open efficacy in human cardioviral infections.
Structured PICO
P
Population
COS-1 cells transfected with expression vectors yielding mono-, di-, and tricistronic mRNAs
I
Intervention
5' nontranslated region (5'NTR) of encephalomyocarditis virus (EMCV) or adenovirus tripartite leader
C
Comparator
Deletion in the EMCV 5'NTR, or capped globin 5'NTR
O
Outcome
Translation of reporter genes (CAT) and resistance to inhibition by poliovirus infectionsurrogate
The study demonstrates that the EMCV 5'NTR contains an internal ribosomal entry site (IRES) enabling cap-independent translation initiation in vivo.
Cite This Study
Jang et al. (1989) studied this question.