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September 1, 1991Journal of VirologyOpen Access

Internal initiation of translation in retroviral vectors carrying picornavirus 5' nontranslated regions

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Population

Mouse NIH 3T3 cells and human and canine skin fibroblasts

Comparison

Retroviral vectors constructed with 5'… vs Control vectors that did not contain the 5' NTR…

Design

Preclinical

Authors

MAMohamed AdamSIMAD UniversityAMA. Dusty MillerBrigham Young UniversityWOWilliam OsborneHeartlands Hospital

Discussion

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Implication

Facilitates bicistronic retroviral vector design; extends prior engineering strategies but remains hypothesis-generating for therapeutic applications.

Structured PICO

P
Population
Mouse NIH 3T3 cells and human and canine skin fibroblasts
I
Intervention
Retroviral vectors constructed with 5' nontranslated region (NTR) sequences from encephalomyocarditis virus and poliovirus
C
Comparator
Control vectors that did not contain the 5' NTR sequences
O
Outcome
Protein expression of downstream coding regions

Inclusion of picornavirus 5' NTR sequences in retroviral vectors allows coordinate expression of two coding regions from a single mRNA in stable cell lines.

Cite This Study

Adam et al. (1991) studied this question.

synapsesocial.com/papers/6a8212646cfba4e31a9fd86chttps://doi.org/10.1128/jvi.65.9.4985-4990.1991
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation1988 · 1,377 citations
  2. 2Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo1989 · 419 citations
  3. 3Internal binding of eucaryotic ribosomes on poliovirus RNA: translation in HeLa cell extracts1989 · 128 citations
  4. 4Cap-Independent Translation of Poliovirus mRNA is Conferred by Sequence Elements within the 5′ Noncoding Region1988 · 136 citations
  5. 5Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.1988 · 271 citations