January 1, 1997Socio-Environmental Systems ModelingOpen Access

Internal entry of ribosomes is directed by the 5' noncoding region of classical swine fever virus and is dependent on the presence of an RNA pseudoknot upstream of the initiation codon

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Population

Bicistronic RNAs containing the 373-nucleotide-long 5' nontranslated region of the classical swine fever…

Comparison

Deletion analysis and site-directed mutagenesis… vs Wild-type RNAs

Design

Preclinical

Authors

RRRené RijnbrandDicerna Pharmaceuticals (United States)TSTahar van der StraatenLeiden University PRPiet A. van RijnNorth-West University

Discussion

Member takes

Implication

Stem II base pairing is essential for CSFV IRES activity; confirms pseudoknot dependence and leaves open targeted antiviral strategies.

Structured PICO

Population

Bicistronic RNAs containing the 373-nucleotide-long 5' nontranslated region (NTR) of the classical swine fever virus (CSFV) genome

Intervention

Deletion analysis and site-directed mutagenesis of the pseudoknot structure at the 3' end of the CSFV 5' NTR

Comparator

Wild-type RNAs

Outcome

Translation activity (IRES-mediated translation)surrogate

The 5' noncoding region of classical swine fever virus directs internal ribosome entry, which depends on an RNA pseudoknot upstream of the initiation codon.

Cite This Study

Rijnbrand et al. (1997) studied this question.

synapsesocial.com/papers/6a82f4e95c109110e17d5620 https://doi.org/10.1128/jvi.71.1.451-457.1997

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Internal entry of ribosomes is directed by the 5' noncoding region of classical swine fever virus and is dependent on the presence of an RNA pseudoknot upstream of the initiation codon

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Internal entry of ribosomes is directed by the 5' noncoding region of classical swine fever virus and is dependent on the presence of an RNA pseudoknot upstream of the initiation codon

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider