Key result
Mutagenesis of the classical swine fever virus IRES revealed that deletions invading domain II reduced activity to 20%, and base pairing in both stems of the pseudoknot is essential for function.
Population
Classical swine fever virus (CSFV) RNA (in vitro transcription and translation model)
Comparison
Mutagenesis of the 5' untranslated region vs Wild-type CSFV RNA sequence
Design
Preclinical
Authors
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Domain II and pseudoknot integrity are required for CSFV IRES activity; hypothesis-generating for antiviral design, needs in vivo confirmation.
Specific structural elements in the 5' UTR of CSFV, including domain II and the pseudoknot, are critical for IRES-mediated translation.
Fletcher et al. (2002) studied Classical swine fever virus (CSFV) infection (in vitro model). Mutagenesis of CSFV IRES vs. Wild-type CSFV IRES was evaluated on IRES activity (translation efficiency). Mutagenesis of the classical swine fever virus IRES revealed that deletions invading domain II reduced activity to 20%, and base pairing in both stems of the pseudoknot is essential for function.
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