Key result
Targeted mutations within domain IIIf of the classical swine fever virus IRES that retain >75% of wild-type translation efficiency resulted in viable mutant viruses with reduced growth in cell culture.
Population
Baby hamster kidney (BHK) cells infected with recombinant vaccinia virus vTF7-3 and porcine PK15 cells
Comparison
Targeted mutations within domains II, IIId, and… vs Wild-type (wt) CSFV strain Paderborn
Design
Preclinical
Authors
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Hypothesis-generating for IRES-targeted CSFV attenuation; leaves open in vivo stability and vaccine potential.
Targeted mutations in the IRES of classical swine fever virus can modulate translation initiation efficiency and reduce viral growth in cell culture.
Friis et al. (2012) studied Classical Swine Fever Virus (CSFV). Targeted mutations within the internal ribosome entry site (IRES) vs. Wild-type (wt) virus was evaluated on Translation initiation efficiency and viral growth in cell culture. Targeted mutations within domain IIIf of the classical swine fever virus IRES that retain >75% of wild-type translation efficiency resulted in viable mutant viruses with reduced growth in cell culture.
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