Key result
A single nucleotide substitution (U to C at position -376) in the IRES of FMDV R100 resulted in a 1.5- to 5-fold increase in cap-independent translation efficiency compared to the C-S8c1 IRES.
Population
BHK-21 cells and other FMDV-sensitive and FMDV-resistant cell lines
Comparison
FMDV R100 IRES and single mutation constructs vs Wild-type FMDV C-S8c1 IRES
Design
Preclinical
Authors
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Supports IRES mutation as FMDV virulence factor; leaves open confirmation in natural infections.
Effect estimate: 1.5- to 5-fold increase
A single point mutation in the IRES element of foot-and-mouth disease virus significantly increases cap-independent translation efficiency, potentially explaining viral hypervirulence.
Martı́nez-Salas et al. (1993) studied Foot-and-mouth disease virus (FMDV) infection (in vitro). Single nucleotide substitution (U to C at position -376) in the IRES of FMDV R100 vs. C-S8c1 IRES (parental virus) was evaluated on IRES-directed cap-independent protein synthesis (luciferase synthesis) (1.5- to 5-fold increase). A single nucleotide substitution (U to C at position -376) in the IRES of FMDV R100 resulted in a 1.5- to 5-fold increase in cap-independent translation efficiency compared to the C-S8c1 IRES.
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