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December 1, 1989Journal of VirologyOpen Access

Attenuation and cell culture adaptation of hepatitis A virus (HAV): a genetic analysis with HAV cDNA

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Population

Monkey kidney cells (in vitro) and marmosets (in vivo model)

Comparison

Transfection with RNA transcripts from chimeric… vs Attenuated and wild-type HAV

Design

Preclinical

Authors

JCJeffrey I. CohenNational Institutes of HealthBRB RosenblumNational Institutes of HealthSFS M FeinstoneUniversity of Maryland, Baltimore

Discussion

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Implication

May inform attenuated HAV vaccine strategies; leaves open translation from animal models to clinical use.

Structured PICO

P
Population
Monkey kidney cells (in vitro) and marmosets (in vivo model)
I
Intervention
Transfection with RNA transcripts from chimeric and mutagenized hepatitis A virus (HAV) cDNAs
C
Comparator
Attenuated and wild-type HAV
O
Outcome
Viral growth in vitro and attenuation in vivosurrogate

The P2-P3 region of the hepatitis A virus genome is critical for both cell culture adaptation and viral attenuation.

Cite This Study

Cohen et al. (1989) studied this question.

synapsesocial.com/papers/6a746cddf5fc394144032357https://doi.org/10.1128/jvi.63.12.5364-5370.1989
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular Cloning of cDNA from Hepatitis A Virus Strain HM-175 after Multiple Passages in vivo and in vitro1986 · 13 citations
  2. 2Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses1987 · 385 citations
  3. 3Molecular cloning and characterization of hepatitis A virus cDNA.1983 · 140 citations
  4. 4Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage1981 · 154 citations
  5. 5Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization1987 · 44 citations