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August 1, 1986Journal of General Virology

Molecular Cloning of cDNA from Hepatitis A Virus Strain HM-175 after Multiple Passages in vivo and in vitro

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Population

Hepatitis A virus (HAV) strain HM-175 passaged six times in marmosets, 59 times in cell culture

Comparison

Molecular cloning of cDNA into the PstI site of… vs HAV strain HM-175 cDNA cloned from viral RNA…

Design

Preclinical

Authors

BRB C RossCSL (United Kingdom)BABruce N. AndersonHarding University Main CampusACA G CoulepisFairfield Infectious Diseases Hospital

Discussion

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Implication

Localizes passaged HAV changes to the 5' noncoding region; leaves open their role in attenuation or human infectivity.

Structured PICO

P
Population
Hepatitis A virus (HAV) strain HM-175 passaged six times in marmosets, 59 times in cell culture
I
Intervention
Molecular cloning of cDNA into the PstI site of plasmid pBR322
C
Comparator
HAV strain HM-175 cDNA cloned from viral RNA after three passages in marmosets
O
Outcome
Restriction endonuclease mapping and DNA sequencing

Molecular cloning and sequencing of HAV strain HM-175 after multiple passages revealed eight base changes in the 5' noncoding region compared to early passage virus.

Cite This Study

Ross et al. (1986) studied this question.

synapsesocial.com/papers/6a708f6c8031ec7bb1dc6d53https://doi.org/10.1099/0022-1317-67-8-1741
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.1975 · 3,479 citations
  2. 2Molecular cloning and partial sequencing of hepatitis A viral cDNA1985 · 75 citations
  3. 3DNA sequencing with chain-terminating inhibitors1977 · 69,512 citations
  4. 4Primary structure and gene organization of human hepatitis A virus.1985 · 200 citations
  5. 5Molecular cloning and characterization of hepatitis A virus cDNA.1983 · 140 citations