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November 1, 1992Journal of VirologyOpen Access

Mutations within the 5' nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells

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Population

Chimeric infectious cDNA clones of human hepatitis A virus in continuous African green monkey kidney cells…

Comparison

Substitution of portions of the 5' nontranslated… vs Wild-type or other chimeric variants

Design

Preclinical

Authors

SDStephen DayGeorgetown UniversityPMPaula C. MurphyUniversity of North Carolina at Chapel HillEBEdwin A. BrownUniversity of North Carolina at Chapel Hill

Discussion

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Implication

No immediate clinical implications from cell-line data; leaves open in vivo relevance of 5'NTR mutations for HAV.

Structured PICO

P
Population
Chimeric infectious cDNA clones of human hepatitis A virus (HAV) in continuous African green monkey kidney (BS-C-1) cells and continuous fetal rhesus monkey kidney (FRhK-4) cells
I
Intervention
Substitution of portions of the 5' nontranslated region (5'NTR) of cell culture-adapted HM175/p35 virus with cDNA from wild-type virus (HM175/wt) or HM175/p16
C
Comparator
Wild-type or other chimeric variants
O
Outcome
Virus replication competence (measured by radioimmunofocus size and one-step growth experiments)surrogate

Mutations at bases 152 and/or 203 to 207 in the 5'NTR of hepatitis A virus enhance replication in a host cell-specific manner.

Cite This Study

Day et al. (1992) studied this question.

synapsesocial.com/papers/6a8212646cfba4e31a9fd86bhttps://doi.org/10.1128/jvi.66.11.6533-6540.1992
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Mutations responsible for adaptation of hepatitis A virus to efficient growth in cell culture1991 · 66 citations
  2. 2Nucleotide Sequence of High-passage Hepatitis A Virus Strain HM175: Comparison with Wild-type and Cell Culture-adapted Strains1989 · 25 citations
  3. 3Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture1992 · 74 citations
  4. 4The 5' nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro1991 · 211 citations
  5. 5Molecular cloning and characterization of hepatitis A virus cDNA.1983 · 140 citations