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December 1, 1999Journal of VirologyOpen Access

Mutations Abrogating the RNase Activity in Glycoprotein Ernsof the Pestivirus Classical Swine Fever Virus Lead to Virus Attenuation

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Population

Piglets and tissue culture models infected with Classical swine fever virus (CSFV)

Comparison

RNase-negative CSFV mutants (mutations at codon… vs RNase-positive control virus (wild-type)

Design

Preclinical

Authors

GMGregor MeyersFriedrich-Loeffler-InstitutASArmin SaalmüllerUniversity of Veterinary Medicine ViennaMBMathias BüttnerLeipzig University

Discussion

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Implication

Codon 346 mutations attenuate CSFV in animal models; leaves open RNase activity as target for live-attenuated vaccines.

Structured PICO

P
Population
Piglets and tissue culture models infected with Classical swine fever virus (CSFV)
I
Intervention
RNase-negative CSFV mutants (mutations at codon 297, 346, or both in glycoprotein E(rns))
C
Comparator
RNase-positive control virus (wild-type)
O
Outcome
Development of classical swine fever symptoms (fever, viremia, decrease of peripheral B cells)

Mutations abrogating the RNase activity in glycoprotein E(rns) of the classical swine fever virus lead to virus attenuation, suggesting a role for RNase activity in viral pathogenicity.

Cite This Study

Meyers et al. (1999) studied this question.

synapsesocial.com/papers/6a703ff922b3175134db4e47https://doi.org/10.1128/jvi.73.12.10224-10235.1999
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Also Consider

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  1. 1Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.1986 · 343 citations
  2. 2Classical Swine Fever Virus Leader Proteinase N pro Is Not Required for Viral Replication in Cell Culture1998 · 93 citations
  3. 3Pathogenesis of mucosal disease: a cytopathogenic pestivirus generated by an internal deletion1994 · 110 citations
  4. 4Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs1997 · 163 citations
  5. 5Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs1996 · 148 citations