A specific 60-nucleotide sequence in the 5'-noncoding region of poliovirus RNA is essential for its translation in vitro.
Identifies a potential antiviral target in poliovirus translation; leaves open confirmation in cellular and in vivo models.
A truncated poliovirus RNA that contains the entire 5'-noncoding region as well as some capsid protein-coding sequences was produced from cloned cDNA inserted into an SP6 transcription vector and subsequently was translated in a mixed rabbit reticulocyte-HeLa cell lysate. Deletions or modifications of regions of the 5'-noncoding sequences had significant effects upon the efficiency of translation. The presence of a 60-nucleotide sequence located at positions 567 to 627 appeared to be essential for active ribosome binding and translation of this uncapped RNA.
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Bieńkowska-Szewczyk et al. (1988) studied this question.
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