July 22, 1988Science
Translation in Mammalian Cells of a Gene Linked to the Poliovirus 5′ Noncoding Region
Population
Mammalian cells
Comparison
Transfection with a construct fusing the… vs Constructs with small lesions throughout region P
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May inform IRES-based vector design; leaves open validation in cardiovascular models.
Structured PICO
P
Population
Mammalian cells
I
Intervention
Transfection with a construct fusing the poliovirus 5' noncoding region (region P) to a reporter gene
C
Comparator
Constructs with small lesions throughout region P
O
Outcome
Expression of the reporter gene (cap-independent initiation of protein synthesis)
The central portion of the poliovirus 5' noncoding region functions as a coherent unit hundreds of nucleotides long to enable cap-independent translation initiation.
Cite This Study
Trono et al. (1988) studied this question.