Key result
Deletion mutants revealed an inhibitory cis-acting element within the 5' noncoding region of poliovirus mRNA that restricts translation in certain extracts but is overcome by a trans-acting factor in HeLa cells.
Identifies an inhibitory cis-acting element in the poliovirus 5' noncoding region and a HeLa cell-specific trans-acting factor that overcomes this translational restriction.
Provides early insights into viral mRNA translation; leaves open relevance to cardiovascular applications.
Poliovirus mRNA contains a long 5' noncoding region of about 750 nucleotides (the exact number varies among the three virus serotypes), which contains several AUG codons upstream of the major initiator AUG. Unlike most eucaryotic mRNAs, poliovirus does not contain a m7GpppX (where X is any nucleotide) cap structure at its 5' end and is translated by a cap-independent mechanism. To study the manner by which poliovirus mRNA is expressed, we examined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of the mRNA. In this paper we report striking translation system-specific differences in the ability of the altered mRNAs to be translated. The results suggest the existence of an inhibitory cis-acting element(s) within the 5' noncoding region of poliovirus (between nucleotides 70 and 381) which restricts mRNA translation in reticulocyte lysate, wheat germ extract, and Xenopus oocytes, but not in HeLa cell extracts. In addition, we show that HeLa cell extracts contain a trans-acting factor(s) that overcomes this restriction.
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Pelletier et al. (1988) studied Poliovirus mRNA translation. Deletion mutants within the 5' noncoding region of poliovirus mRNA vs. Wild-type or other mutants was evaluated on Translational efficiency in different cell extracts. Deletion mutants revealed an inhibitory cis-acting element within the 5' noncoding region of poliovirus mRNA that restricts translation in certain extracts but is overcome by a trans-acting factor in HeLa cells.
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