5'-terminal structure of poliovirus polyribosomal RNA is pUp.
Key result
Poliovirus RNA purified from virus-specific polyribosomes lacks a 5'-terminal m7G and instead contains pUp, indicating that a 5'-terminal m7G is not required for translation in animal cells.
Authors
Discussion
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Member takes
Implication
Questions necessity of m7G cap for translation; leaves open cap-independent mechanisms in animal cells.
PICO
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Population
In vitro study of HeLa cells infected with poliovirus to determine the 5'-terminal structure of viral mRNA.
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Intervention / Comparator
Poliovirus infection (Multiplicity of infection of 20)
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Primary Outcome
5'-terminal structure of poliovirus polyribosomal RNA
Limitations
- The exact end of poliovirus virion RNA remains to be determined.
- It is unclear how the synthesis of the RNA initiates without a triphosphate at its 5'-terminus.
Cite This Study
Hewlett et al. (1976) studied Poliovirus infection (in vitro). Poliovirus infection was evaluated on 5'-terminal structure of poliovirus polyribosomal RNA. Poliovirus RNA purified from virus-specific polyribosomes lacks a 5'-terminal m7G and instead contains pUp, indicating that a 5'-terminal m7G is not required for translation in animal cells.