February 1, 1976Proceedings of the National Academy of Sciences242 citationsOpen Access
5'-terminal structure of poliovirus polyribosomal RNA is pUp.
Key Result
Poliovirus RNA purified from virus-specific polyribosomes lacks a 5'-terminal m7G and instead contains pUp, indicating that a 5'-terminal m7G is not required for translation in animal cells.
PICO
P
Population
In vitro study of HeLa cells infected with poliovirus to determine the 5'-terminal structure of viral mRNA.
I
Intervention / Comparator
Poliovirus infection (Multiplicity of infection of 20)
O
Primary Outcome
5'-terminal structure of poliovirus polyribosomal RNA
Limitations
- The exact end of poliovirus virion RNA remains to be determined.
- It is unclear how the synthesis of the RNA initiates without a triphosphate at its 5'-terminus.
Abstract
Poliovirus RNA purified from virus-specific polyribosomes does not contain m7G in a 5'-5'-pyrophosphate linkage at its 5'-end. The only potential 5'-end found in ribonuclease digests of this RNA is pUp, which is present in a yield of 1 mole/mole of poliovirus RNA. We conclude that a 5'-terminal m7G is not required for translation of at least one RNA species in animal cells.
Cite This Study
Hewlett et al. (Sun,) conducted a other in Poliovirus infection (in vitro). Poliovirus infection was evaluated on 5'-terminal structure of poliovirus polyribosomal RNA. Poliovirus RNA purified from virus-specific polyribosomes lacks a 5'-terminal m7G and instead contains pUp, indicating that a 5'-terminal m7G is not required for translation in animal cells.