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November 1, 1991Journal of VirologyOpen Access

Terminal 7-methyl-guanosine cap structure on the normally uncapped 5' noncoding region of poliovirus mRNA inhibits its translation in mammalian cells

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Population

HeLa cells (human tissue culture cells) and in vitro translation systems

Comparison

In vitro-transcribed hybrid RNA molecules… vs Similar transcripts without a terminal cap…

Design

Preclinical

Authors

SHSimon J. HambidgeDenver Health Medical CenterPSPeter SarnowStanford University

Discussion

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Implication

Cellular cap inhibition of poliovirus translation cautions against in vitro extrapolation; leaves open cellular factor identification.

Structured PICO

P
Population
HeLa cells (human tissue culture cells) and in vitro translation systems
I
Intervention
In vitro-transcribed hybrid RNA molecules containing a capped 5' noncoding region of poliovirus linked to the coding region of the firefly luciferase gene
C
Comparator
Similar transcripts without a terminal cap structure
O
Outcome
Luciferase accumulation/translation levelssurrogate

The presence of a 5'-terminal cap structure inhibits the translation of poliovirus mRNA in intact mammalian cells but not in in vitro systems, highlighting differences in cap-independent translation factors.

Cite This Study

Hambidge et al. (1991) studied this question.

synapsesocial.com/papers/6a8306228b169f476ee8de18https://doi.org/10.1128/jvi.65.11.6312-6315.1991
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.1976 · 277 citations
  2. 2Translational efficiency of poliovirus mRNA: mapping inhibitory cis-acting elements within the 5' noncoding region1988 · 67 citations
  3. 3An RNA hairpin at the extreme 5' end of the poliovirus RNA genome modulates viral translation in human cells1991 · 102 citations
  4. 4Cap-Independent Translation of Poliovirus mRNA is Conferred by Sequence Elements within the 5′ Noncoding Region1988 · 136 citations
  5. 5Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo1989 · 419 citations