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February 1, 1991Journal of VirologyOpen Access

An RNA hairpin at the extreme 5' end of the poliovirus RNA genome modulates viral translation in human cells

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Key result

A mutated RNA hairpin at the extreme 5' end of the poliovirus genome (1-5NC-S21) caused a fivefold decrease in viral translation relative to wild-type-infected human cells.

Population

Human cells (HeLa cells) and infectious poliovirus cDNA clones

Comparison

Mutated poliovirus cDNA vs Wild-type poliovirus

Design

Preclinical

Authors

ESE A SimoesColorado School of Public HealthPSPeter SarnowStanford University

Discussion

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Implication

Most poliovirus 5' or capsid mutations abolish infectivity; leaves open functional genome mapping in replication studies.

Structured PICO

P
Population
Human cells (HeLa cells) and infectious poliovirus cDNA clones
I
Intervention
Mutated poliovirus cDNA (1-5NC-S21 bearing a 6-nucleotide insertion in the loop of a stable RNA hairpin at the 5' end)
C
Comparator
Wild-type poliovirus
O
Outcome
Viral translation efficiencysurrogate

Main Result

Effect estimate: fivefold decrease

An RNA hairpin located at the extreme 5' end of the viral RNA profoundly affects the translation efficiency of poliovirus RNA in infected cells.

Cite This Study

Simoes et al. (1991) studied Poliovirus infection. Mutated RNA hairpin (1-5NC-S21) vs. Wild-type poliovirus was evaluated on Viral translation efficiency (fivefold decrease). A mutated RNA hairpin at the extreme 5' end of the poliovirus genome (1-5NC-S21) caused a fivefold decrease in viral translation relative to wild-type-infected human cells.

synapsesocial.com/papers/6a6c8be726a7f98052db42b0https://doi.org/10.1128/jvi.65.2.913-921.1991
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1An RNA sequence of hundreds of nucleotides at the 5' end of poliovirus RNA is involved in allowing viral protein synthesis1988 · 173 citations
  2. 2Firefly luciferase gene: structure and expression in mammalian cells.1987 · 3,093 citations
  3. 3Construction of viable deletion and insertion mutants of the Sabin strain of type 1 poliovirus: function of the 5' noncoding sequence in viral replication1987 · 146 citations
  4. 4Genetic complementation among poliovirus mutants derived from an infectious cDNA clone1986 · 129 citations
  5. 5Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.1986 · 343 citations