Key result
Poliovirus revertants with an eight-nucleotide lesion in stem-loop V displayed decreased translation initiation efficiency and reduced infectious virus yields in neuronal cells compared to HeLa cells.
Population
HeLa cells and neuronal cells (in vitro translation studies and cell-free systems)
Comparison
Poliovirus revertants with stem-loop lesions vs Wild-type poliovirus / HeLa cell extracts
Design
Preclinical
Authors
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Neuronal-specific attenuation may aid live-vaccine design; leaves open human translation and in vivo confirmation.
RNA secondary structures within the poliovirus internal ribosome entry site interact with neuronal cell factors to direct translation initiation, contributing to the attenuation phenotype.
Haller et al. (1996) studied Poliovirus infection. X472 poliovirus revertants (X472R1 and X472R2) vs. Wild-type poliovirus / HeLa cell extracts was evaluated on Translation initiation efficiency and infectious virus particle yields. Poliovirus revertants with an eight-nucleotide lesion in stem-loop V displayed decreased translation initiation efficiency and reduced infectious virus yields in neuronal cells compared to HeLa cells.
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