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March 1, 1992Journal of VirologyOpen Access

Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation

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Population

Cell-free reactions programmed with cDNA-derived RNA transcripts of encephalomyocarditis virus and other…

Comparison

Mapping of RNA elements and addition of small 5'… vs Exogenous capped or uncapped mRNAs

Design

Preclinical

Authors

GDG M DukeUniversity of Wisconsin–MadisonMHMichael A. HoffmanUnited Christian HospitalAPAnn C. PalmenbergUniversity of Wisconsin–Madison

Discussion

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Implication

May guide IRES-targeted antivirals for EMCV; hypothesis-generating in animal models and leaves open clinical relevance.

Structured PICO

P
Population
Cell-free reactions programmed with cDNA-derived RNA transcripts of encephalomyocarditis virus (EMCV-Rueckert) and other poly(C)-containing cardioviruses (mengovirus, EMCV-B, and EMCV-D)
I
Intervention
Mapping of RNA elements (stem-loop motifs I, J, K, H) and addition of small 5' nontranslated region fragments (bases 667 to 797)
C
Comparator
Exogenous capped or uncapped mRNAs (for competitive inhibition assays)
O
Outcome
Translation of EMCV proteins / translational activitysurrogate

Stem-loop motifs I, J, and K in the 5' nontranslated region of EMCV are critical for cap-independent translation and may competitively downregulate host protein synthesis.

Cite This Study

Duke et al. (1992) studied this question.

synapsesocial.com/papers/6a6fcb4aac440176ef287476https://doi.org/10.1128/jvi.66.3.1602-1609.1992
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region1984 · 234 citations
  2. 2Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the P3 region1986 · 121 citations
  3. 3Functional analysis of the internal translation initiation site of foot-and-mouth disease virus1990 · 203 citations
  4. 4Shutoff of host translation by encephalomyocarditis virus infection does not involve cleavage of the eucaryotic initiation factor 4F polypeptide that accompanies poliovirus infection1985 · 61 citations
  5. 5An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates1976 · 3,971 citations