Stage-specific ecotoxicological mechanisms in Artemia: Physiological disruption, immune disorder, and gut microbiota disturbance in response to nanoplastics stress
Randomized trial investigates the impact of nanoplastics on Artemia's physiological and microbial health across developmental stages, indicating significant ecological risks.
Key Points
This study aims to explore the specific ecotoxicological mechanisms of nanoplastics on Artemia during different developmental stages.
Utilized Artemia to assess effects of 100 nm polystyrene nanoplastics at various concentrations (0, 0.5, 5, 10 µg/mL).
Analyzed responses including oxidative stress, immune responses, and gut microbiota across developmental stages: CN, NM, MJ.
Applied 16S rRNA sequencing to evaluate gut microbiota changes.
PSNPs induced acute ROS and MDA accumulation in the CN stage, indicating oxidative damage.
The NM stage exhibited significant inflammatory response and reduced feeding and growth.
Gut microbiota analysis revealed stage-specific dysbiosis, with the NM stage showing sustained decline.