Biocontrol Potential of Arthrobotrys thaumasius Isolated from Banana Roots (Musa spp. L.) Against Root-Knot Nematodes of the Genus Meloidogyne

Root-knot nematodes of the genus Meloidogyne are among the most destructive endoparasitic nematodes due to their ability to infect a wide range of agriculturally important crops. In this context, nematode-trapping fungi have been widely recognized for their potential as biological control agents against plant-parasitic nematodes. In the present study, the nematode-trapping fungus Arthrobotrys thaumasius was isolated from roots soils of banana plants (Musa spp.) in the canton of Guayaquil, Guayas Province, Ecuador. Four strains were obtained and identified based on sequence analyses of molecular markers. In addition, the in vitro growth and sporulation of the isolates were evaluated, with cornmeal agar and oat agar proving to be the most suitable culture media. Three A. thaumasius isolates exhibited attraction and capture rates exceeding 65% against second-stage juveniles (J2) of Meloidogyne. This study represents the first report of the isolation and characterization of A. thaumasius in Ecuador and demonstrates that isolates HN-20, HN-21, and HN-24 have high potential as biological control agents, positioning them as promising candidates for the sustainable management of root-knot diseases caused by Meloidogyne spp.