Gonadal Sex and Sex-Chromosome Complement Interact to Affect Ethanol Consumption in Adolescent Four Core Genotypes Mice

Figures

• 

  Figure 1. Mean per-session bout Parameters as a function of ethanol concentration. Figures plot mean values (error bars represent ±SEM) of per-session bout parameters for each ethanol concentration (20%, 10% or 0%) that are grouped by GS (FEMALE [i.e., with ovaries] or MALE [i.e., with testes]) and split SCC: XX or XY) (left side of each letter) or vice versa (right side of each letter) so as to better visualize simple effects of GS and SCC within levels of the 2nd genotype factor. These parameters include the Sum of Bout Amounts ((A), g/kg/h), Sum of Bout Durations ((B), s/h), Bout Count (C), Mean Bout Consumption Rate ((D), g/kg/h/bout; within-bout rates), 1st Bout Latency ((E), minutes from session start), Maximum Bout Amount ((F), g/kg/h/bout), Maximum Bout Duration ((G), s/h/bout), and Maximum Bout Consumption Rate ((H), g/kg/h/bout; within-bout rate). Sidak-adjusted significance values for simple effects of SCC and GS: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Sidak-adjusted significance values for simple effects of ethanol concentration within genotype use the same asterisk coding for p-values, but are color coded to indicate genotype group and indicate ethanol…

• 

  Figure 2. Mean per-session ethanol bout parameters as a function of (within-session) time. (A–D): Figures plot mean values (error bars represent ±SEM) of time-binned bout parameters for ethanol consumption (20% + 10% ethanol solutions) that are grouped by GS (FEMALE [i.e., with ovaries] or MALE [i.e., with testes]) and split by SCC: XX or XY) (left side of each letter) or vice versa (right side of each letter) so as to better visualize simple effects of GS and SCC within levels of the 2nd factor. These per-bin parameters include the Sum of Ethanol Bout Amounts ((A), g/kg), Sum of Ethanol Bout Durations ((B), s), Count of Ethanol Bouts (C), and Mean Ethanol Bout Consumption Rate ((D), g/kg/h; within-bout rates). Sidak-adjusted significance values for simple effects of SCC and GS: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Sidak-adjusted significance values for simple effects of time bin within genotype use the same asterisk coding for p-values, but are color coded to indicate genotype group and numerically indicate the session from which that session differs (e.g., ≠80* in purple represents a significant difference from the 80 min time bin in XX mice).

• 

  Figure 3. BECs as a function of ethanol consumed. Blood samples were collected within 5 min of the completion of the last session; and BECs (mg/dL) are plotted against ethanol consumption (g/kg BW/h). No statistically significant main effect of either GS or SCC, nor of their interaction, was found for BECs (ANCOVA, all p > 0.13), but there was a main effect of EtOH (p < 0.000001) (Spearman’s rho for BEC vs. EtOH: rs(33) = 0.79). The equation for the line is Y(BEC) = −8.4 + 67 × X(EtOH). Group n values for BECs: XXF = 11, XXM = 7, XYF = 6, XYM = 9.