Dynamic Regulation of HIF1α and Oxygen-Sensing Factors in Cyclic Bovine Corpus Luteum and During LPS Challenge

Figures

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  Figure 1. Relative mRNA expression of HIF1A (HIF1α) and HIF1α-regulating factors, i.e., EGLN2 (PHD1), EGLN1 (PHD2), EGLN3 (PHD3), HIF1AN (FIH), and VHL in bovine CL during early, mid-, and late luteal stages. Relative gene expression as determined by semi-quantitative real-time (TaqMan) PCR is presented as mean ± standard error of mean (±SEM). Bars with asterisks differ at * p < 0.05 and ** p < 0.01.

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  Figure 2. Luteal localization of transcripts (mRNA) encoding for HIF1A (HIF1α) (A,B), EGLN2 (PHD1) (C,D), EGLN1 (PHD2) (E), EGLN3 (PHD3) (F,G), and VHL (H,I) in bovine CL, shown at mid-luteal stage. Black arrow: large luteal cells; grey arrow: small luteal cells; arrowhead: vessel. Explanations in text. The sense probes were employed as negative controls (smaller inserts at the same magnification to (B,D,E,G,I)).

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  Figure 3. Relative mRNA expression of HIF1A (HIF1α) and its regulatory factors, EGLN2 (PHD1), EGLN1 (PHD2), EGLN3 (PHD3), HIF1AN (FIH), and VHL during and after E. coli-LPS treatment. Relative gene expression as determined by semi-quantitative real-time (TaqMan) PCR is presented as mean ± standard error of mean (±SEM). Bars with asterisks differ at * p < 0.05 and ** p < 0.01.

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  Figure 4. Relative mRNA expression of ICAM1 and NFKB2 during and after E. coli-LPS treatment. Relative gene expression as determined by semi-quantitative real-time (TaqMan) PCR is presented as mean ± standard error of mean (±SEM). Bars with asterisks differ at * p < 0.05, ** p < 0.01, and *** p < 0.001.

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  Figure 5. Schematic representation of the involvement of HIF1α and oxygen-sensing factors in the cyclic bovine corpus luteum, as well as their expression in the CL of cows treated with lipopolysaccharide (LPS; gram-negative bacterial endotoxin). The data regarding STAR derive from [32]. This figure was created with BioRender.com.