The MgATP-Dependent Protein Phosphatase and Protein Phosphatase 1 Have Identical Substrate Specificities
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Implication
Randomized trial evaluates the substrate specificity of MgATP-dependent phosphatase in muscle tissue, suggesting shared characteristics with protein phosphatase 1.
Key Points
- The study investigates the substrate specificities of MgATP-dependent protein phosphatase and compares it with protein phosphatase 1.
- Conducted enzymatic assays on phosphoproteins using MgATP and activating factor FA.
- Measured activities of MgATP-dependent phosphatase and protein phosphatase 1 on various substrates, including phosphorylase kinase and glycogen synthase.
- Analyzed effects of inhibitors on both enzymes to assess similarities.
- Both dephosphorylated the beta-subunit of phosphorylase kinase 40-fold faster than the alpha-subunit, with similar relative activities.
- Demonstrated similar inhibition by inhibitor 1 and inhibitor 2 in both enzymes.
- Proposed that MgATP-dependent protein phosphatase is a type-1 protein phosphatase, distinct from type-2 protein phosphatases.
Cite This Study
Stewart et al. (2005) studied this question.