Isolated Purkinje fibers provide a valuable in vitro model for assessing the proarrhythmic risk of new pharmacological compounds.
May aid preclinical drug safety screening; leaves open clinical translation of Purkinje fiber findings.
The proarrhythmic potential of new drugs can be investigated using in vitro electrophysiological techniques measuring the cardiac action potential in isolated Purkinje fibers. Different types of arrhythmias may occur, as early after‐depolarizations (EADs), which are favored by action potential duration lengthening and bradycardia, or delayed after‐depolarizations (DADs), which are facilitated by tachycardia. The effects of a test compound on the occurrence of these arrhythmias, known to induce in vivo torsades de pointes (or triggered activities), can be studied following the experimental protocols described in this unit.
No takes yet. Share an insight, caveat, or question.
Rouet et al. (2001) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: