Curcumin activation of the Nrf2/HO-1 signaling pathway exerted myocardial protection by reducing ROS formation, increasing cardiomyocyte viability, and inhibiting apoptosis in diabetic cardiomyopathy.
Does curcumin reduce ROS formation and improve cardiomyocyte viability in in vitro and in vivo models of diabetic cardiomyopathy?
Curcumin protects against diabetic cardiomyopathy injury by activating the Nrf2/HO-1 signaling pathway and reducing reactive oxygen species.
The hallmark feature of Diabetes mellitus (DM) is hyperglycemia which can lead to excess production of reactive oxygen species (ROS) in the myocardium, contributing to diabetic cardiomyopathy (DCM). Nuclear factor erythroid2-related factor2 (Nrf2), a transcriptional activator, enhances its ability to resist oxidative stress by activating multiple downstream anti-oxidants, anti-inflammatory proteins, and detoxifying enzymes. However, the mechanism of Nrf2 signaling in HG-induced DCM is unclear. In this study, we used HG pretreated H9c2 cells as the experimental basis in vitro, and established a high fat-diet, streptozotocin (STZ) induced Type 2 diabetic rat model in vivo. Meanwhile, we used shRNA-Nrf2 and curcumin (CUR) (as an activator) to affect H9c2 cells, to verify the role of the Nrf2 signaling pathway in DCM. The results showed that the excessive production of ROS caused by HG, which could inhibit the activation of Nrf2-related signaling, resulting in a decrease in cell energy metabolism and an increase in cell apoptosis. Surprisingly, we found that the activation of the Nrf2 signaling pathway significantly increased cardiomyocyte viability, reduced ROS formation, increased antioxidant enzyme activity, and inhibited cardiomyocyte apoptosis. In conclusion, these findings conclusively infer that CUR activation of the Nrf2/HO-1 signaling pathway exerts myocardial protection by reducing ROS formation.
Wu et al. (Tue,) conducted a other in Diabetic cardiomyopathy. Curcumin was evaluated on ROS formation, cardiomyocyte viability, and apoptosis. Curcumin activation of the Nrf2/HO-1 signaling pathway exerted myocardial protection by reducing ROS formation, increasing cardiomyocyte viability, and inhibiting apoptosis in diabetic cardiomyopathy.