February 1, 1960Proceedings of the National Academy of SciencesOpen Access

The Effects of Proteases on the Tropocollagen Macromolecule and on Its Aggregation Properties

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Population

Calf-skin collagen solutions (tropocollagen macromolecules)

Comparison

Proteases (trypsin, pepsin, collagenase) vs Untreated collagen solutions

Design

Preclinical

Authors

AHAlan J. HodgeMarine Biological Laboratory JHJohn H. HighbergerDartmouth–Hitchcock Medical Center GDGottfried G.J. DeffnerLeibniz-Institute for Food Systems Biology at the Technical University of Munich

Discussion

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Implication

Hypothesis-generating for protease modulation of cardiac collagen; leaves open effects on myocardial mechanics in vivo.

Structured PICO

Population

Calf-skin collagen solutions (tropocollagen macromolecules)

Intervention

Proteases (trypsin, pepsin, collagenase)

Comparator

Untreated collagen solutions

Outcome

Depolymerization of linear polymers and modification of end-to-end polymerization properties

Proteases like trypsin and pepsin cleave terminal peptide chains of tropocollagen, preventing end-to-end polymerization without disrupting the triple-helix structure.

Limitations

The enzyme used was not electrophoretically pure, meaning proteolytic activity may not be due to trypsin alone.

Cite This Study

Hodge et al. (1960) studied this question.

synapsesocial.com/papers/6a22ad2c37cd05c4adcd50fb https://doi.org/10.1073/pnas.46.2.197

View Full Paper

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The Effects of Proteases on the Tropocollagen Macromolecule and on Its Aggregation Properties

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

The Effects of Proteases on the Tropocollagen Macromolecule and on Its Aggregation Properties

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider