Key result
A-to-I RNA base editing offers a programmable, tunable, and reversible strategy for cellular engineering and an attractive therapeutic alternative for correcting point mutations.
Why the study?
Targeted transcriptome engineering offers a complementary, tunable, and reversible strategy for cellular engineering, but guidance is needed for selecting and implementing appropriate RNA editing systems for research and therapeutic applications.
This perspective provides a guide on five characterized A-to-I RNA base editing systems for potential therapeutic applications, such as the correction of point mutations.
May guide RNA editing tool selection for research; leaves open clinical translation pending validation trials.
Targeted transcriptome engineering, in contrast to genome engineering, offers a complementary and potentially tunable and reversible strategy for cellular engineering. In this regard, adenosine to inosine (A-to-I) RNA base editing was recently engineered to make programmable base conversions on target RNAs. Similar to the DNA base editing technology, A-to-I RNA editing may offer an attractive alternative in a therapeutic setting, especially for the correction of point mutations. This Perspective introduces five currently characterized RNA editing systems and serves as a reader's guide for implementing an appropriate RNA editing strategy for applications in research or therapeutics.
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Chen et al. (2019) conducted a review in Point mutations. RNA-Guided Adenosine Deaminases (A-to-I RNA editing) was evaluated. A-to-I RNA base editing offers a programmable, tunable, and reversible strategy for cellular engineering and an attractive therapeutic alternative for correcting point mutations.
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