Key result
A-to-I RNA base editing offers a programmable, tunable, and reversible strategy for cellular engineering and an attractive therapeutic alternative for correcting point mutations.
Why the study?
Targeted transcriptome engineering offers a complementary, tunable, and reversible strategy for cellular engineering, but guidance is needed for selecting and implementing appropriate RNA editing systems for research and therapeutic applications.
This perspective provides a guide on five characterized A-to-I RNA base editing systems for potential therapeutic applications, such as the correction of point mutations.
Answers are grounded in this paper and cite their sources.
May guide RNA editing tool selection for research; leaves open clinical translation pending validation trials.
Targeted transcriptome engineering, in contrast to genome engineering, offers a complementary and potentially tunable and reversible strategy for cellular engineering. In this regard, adenosine to inosine (A-to-I) RNA base editing was recently engineered to make programmable base conversions on target RNAs. Similar to the DNA base editing technology, A-to-I RNA editing may offer an attractive alternative in a therapeutic setting, especially for the correction of point mutations. This Perspective introduces five currently characterized RNA editing systems and serves as a reader's guide for implementing an appropriate RNA editing strategy for applications in research or therapeutics.
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Chen et al. (2019) conducted a review in Point mutations. RNA-Guided Adenosine Deaminases (A-to-I RNA editing) was evaluated. A-to-I RNA base editing offers a programmable, tunable, and reversible strategy for cellular engineering and an attractive therapeutic alternative for correcting point mutations.
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