What is the clinical evidence from this study?

Population: Normal and hypophysectomized rats. Intervention: Thyroid hormone vs. Controls. Primary outcome: Relative amount of mRNA for skeletal and cardiac alpha-actin.

What question did this study set out to answer?

The study aims to explore how thyroid hormone affects the accumulation of skeletal and cardiac alpha-actin mRNA in rat hearts, particularly in hypophysectomized subjects.

April 1, 1990Proceedings of the National Academy of SciencesOpen Access

Effect of thyroid hormone on the accumulation of mRNA for skeletal and cardiac alpha-actin in hearts from normal and hypophysectomized rats.

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Key result

Thyroid hormone administration caused a rapid increase in skeletal alpha-actin mRNA relative to controls (>7 times in normal rats and 15 times in hypophysectomized rats).

Population

Normal and hypophysectomized rats

Comparison

Thyroid hormone vs Controls

Design

Preclinical

Follow-up

2 to 24 hours

Authors

SWSaul WinegradRutgers, The State University of New Jersey CWC WisnewskyInserm KSK. SchwartzStanford Medicine

Discussion

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Implication

No immediate clinical implications for thyroid therapy in heart disease; leaves open actin isoform roles in human cardiac growth.

Key Points

The study aims to explore how thyroid hormone affects the accumulation of skeletal and cardiac alpha-actin mRNA in rat hearts, particularly in hypophysectomized subjects.
Administered thyroid hormone to normal and hypophysectomized rats in doses of 1 or 5 micrograms per 10 g body weight.
Measured mRNA levels for skeletal and cardiac alpha-actin using primer extension.
Animals were sacrificed at 2 to 24 hours post-injection to assess mRNA accumulation.
Skeletal alpha-actin mRNA increased over 7 times in normal rat hearts and 15 times in hypophysectomized hearts post-thyroid hormone administration.
Cardiac alpha-actin mRNA showed a modest increase in both groups.
Findings highlight distinct patterns of activation for actin and myosin heavy chains during cardiac growth.

Structured PICO

Population

Normal and hypophysectomized rats treated with thyroid hormone and sacrificed 2 to 24 hours post-injection.

Intervention

Thyroid hormone (1 or 5 micrograms per 10 g of body weight)

Comparator

Controls

Outcome

Relative amount of mRNA for skeletal and cardiac alpha-actin measured by primer extensionsurrogate

Thyroid hormone induces a rapid increase in skeletal alpha-actin mRNA in rat hearts, demonstrating independent activation patterns of actin and myosin heavy chain multigene families during cardiac growth.

Cite This Study

Winegrad et al. (1990) studied Normal and hypophysectomized rats. Thyroid hormone vs. Controls was evaluated on Relative amount of mRNA for skeletal and cardiac alpha-actin. Thyroid hormone administration caused a rapid increase in skeletal alpha-actin mRNA relative to controls (>7 times in normal rats and 15 times in hypophysectomized rats).

synapsesocial.com/papers/6a22e0d7f940156bc9c4697b https://doi.org/10.1073/pnas.87.7.2456

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Effect of thyroid hormone on the accumulation of mRNA for skeletal and cardiac alpha-actin in hearts from normal and hypophysectomized rats.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effect of thyroid hormone on the accumulation of mRNA for skeletal and cardiac alpha-actin in hearts from normal and hypophysectomized rats.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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