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This study aims to investigate the synthesis of Factor VIII antigen by cultured guinea pig megakaryocytes.

Synthesis of Factor VIII Antigen by Cultured Guinea Pig Megakaryocytes

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This study aims to investigate the synthesis of Factor VIII antigen by cultured guinea pig megakaryocytes.
Factor VIII antigen was immunoprecipitated from guinea pig plasma using rabbit anti-human Factor VIII.
Monospecific antisera to guinea pig Factor VIII were produced and used to detect antigen in both plasma and cultured megakaryocytes.
Radioactive leucine labeling identified and characterized the synthesized Factor VIII antigen via SDS-PAGE.
Cultured guinea pig megakaryocytes synthesized Factor VIII antigen with a polypeptide subunit of 200,000 daltons.
The immunoprecipitated Factor VIII antigen inhibited von Willebrand factor activity in guinea pig plasma.
96.2% of radioactivity from leucine labeling was localized in megakaryocytes.

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Randomized trial shows synthesis of Factor VIII antigen in megakaryocytes, indicating their role in coagulation.

Nachman et al. (1977) studied this question.

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