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November 15, 1991Proceedings of the National Academy of SciencesOpen Access

Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates.

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Population

Genomic RNA from the human prototype strain H of the hepatitis C virus (HCV-H)

Comparison

Molecular cloning and sequencing vs American isolate HCV-1 and Japanese isolates…

Design

Preclinical

Key result

Genomic sequencing of the hepatitis C virus prototype strain H revealed 96% amino acid identity with the American isolate HCV-1 but only 84.9% with Japanese isolates.

Authors

GIGeneviève InchauspéSZS L ZebedeeDLD H Lee

Discussion

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Overview

HCV heterogeneity may require adjusted diagnostics; leaves open subtype effects on outcomes and vaccines.

Structured PICO

P
Population
Genomic RNA from the human prototype strain H of the hepatitis C virus (HCV-H)
E
Exposure
Molecular cloning and sequencing
C
Comparator
American isolate HCV-1 and Japanese isolates HCV-J and HCV-BK
O
Outcome
Genomic sequence and amino acid identitysurrogate

The cloning and sequencing of HCV-H reveals significant heterogeneity between American and Japanese isolates, suggesting the existence of at least two genomic subtypes of HCV.

Cite This Study

Inchauspé et al. (1991) studied Hepatitis C virus. Genomic sequencing of HCV-H vs. American and Japanese isolates (HCV-1, HCV-J, HCV-BK) was evaluated on Genomic sequence and amino acid identity. Genomic sequencing of the hepatitis C virus prototype strain H revealed 96% amino acid identity with the American isolate HCV-1 but only 84.9% with Japanese isolates.

synapsesocial.com/papers/6a22fa1f3bf314e836cb1b09https://doi.org/10.1073/pnas.88.22.10292
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