A comparison of activities associated with Escherichia coli polymerase I, calf thymus 6 to 8 S DNA polymerase and calf thymus 3.4 S DNA polymerase demonstrates that neither exonuclease activity nor pyrophosphorolysis is essential for polymerization. The 3.4 S enzyme has no demonstrable 3'- to 5'-exonuclease activity and cannot carry out pyrophosphate exchange. Under these conditions phosphodiester bond formation is kinetically irreversible.
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Chang et al. (1973) studied this question.
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