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March 21, 2011PLoS ONEOpen Access

Complete Sequencing of pNDM-HK Encoding NDM-1 Carbapenemase from a Multidrug-Resistant Escherichia coli Strain Isolated in Hong Kong

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Authors

PHPak‐Leung HoHospital AuthorityWLWai U. LoUniversity of Hong KongMYMan Kiu YeungUniversity of Hong Kong

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Implication

Randomized trial outlines the genetic characteristics of NDM-1 in E. coli, suggesting its complex evolution and multiple transmission pathways.

Key Points

  • This study aims to characterize the first NDM-1 producing E. coli strain isolated in Hong Kong and analyze the associated plasmid.
  • Plasmid pNDM-HK was sequenced and characterized in detail.
  • The isolate was recovered from clinical samples in Hong Kong.
  • Analysis included sequence alignment and transferability of the plasmid to other E. coli strains.
  • Plasmid pNDM-HK was 88803 bp and transferred to E. coli J53 at high frequencies.
  • It showed 97% homology with pEL60 from Erwina amylovora and contained multiple antibiotic resistance genes.
  • The variable region of the plasmid included composite transposon-like structures linked to resistance mechanisms.

Cite This Study

Ho et al. (2011) studied this question.

synapsesocial.com/papers/6a236379949000401022328chttps://doi.org/10.1371/journal.pone.0017989
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