Key result
In rat hearts subjected to 20 minutes of ischemia, 1 mM amiloride significantly attenuated the rise in cytosolic free calcium (1.0 vs 2.3 microM) and sodium compared to untreated hearts.
Why the study?
Does amiloride reduce the ischemia-induced rise in cytosolic free calcium in perfused rat hearts?
Population
Perfused rat hearts loaded with 5F-BAPTA
Comparison
Amiloride 1 mM vs Absence of amiloride (untreated hearts)
Design
Preclinical
Follow-up
20 minutes
Authors
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Implicates Na-Ca exchange in rat ischemia-induced calcium overload; hypothesis-generating for translation to human cardioprotection.
Does amiloride reduce the ischemia-induced rise in cytosolic free calcium in perfused rat hearts?
Absolute Event Rate: 1% vs 2.3%
Amiloride attenuates the rise in intracellular sodium and calcium during ischemia in rat hearts, supporting the role of Na-Ca exchange in ischemia-induced calcium overload.
Murphy et al. (1991) studied Ischemia. Amiloride vs. Untreated hearts was evaluated on Time-averaged cytosolic free calcium (Cai) after 20 minutes of ischemia. In rat hearts subjected to 20 minutes of ischemia, 1 mM amiloride significantly attenuated the rise in cytosolic free calcium (1.0 vs 2.3 microM) and sodium compared to untreated hearts.
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