Performance and Cost Analysis of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Routine Identification of Yeast

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was compared to phenotypic testing for yeast identification. MALDI-TOF mass spectrometry yielded 96. 3% and 84. 5% accurate species level identifications (spectral scores, ≥ 1. 8) for 138 common and 103 archived strains of yeast. MALDI-TOF mass spectrometry is accurate, rapid (5. 1 min of hands-on time/identification), and cost-effective (0. 50/sample) for yeast identification in the clinical laboratory.