An Evaluation Model for Brain Ischemia Protection in Mice by Low-Intensity Pulsed Ultrasound Stimulation Based on Functional Cortico-Muscular Coupling

Q: What are the key findings of this study?

LIPUS significantly improved DTW-100 scores in both time and frequency domains compared to baseline in BCAO mice (p<0.001), indicating neuroprotective functional preservation.

Q: What is the clinical evidence from this study?

Population: Ischemic Stroke (n=39). Intervention: Low-intensity pulsed ultrasound stimulation (LIPUS) vs. Pre-stimulation BCAO state. Primary outcome: Neuromuscular functional coupling evaluated via dynamic time warping (DTW-100) scoring system (p=<0.001).

Q: What does this research mean for the field?

A novel neuromuscular functional coupling-based dynamic time warping (DTW) model can successfully quantify the neuroprotective effects of low-intensity pulsed ultrasound stimulation (LIPUS) in ischemic stroke. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Ziqiang Jin; Xiaoling Chen; Zhenhua Du; Yi Yuan; Xiaoli Li; Ping Xie

doi:10.3390/bioengineering12050541

May 17, 2025BioengineeringOpen Access

An Evaluation Model for Brain Ischemia Protection in Mice by Low-Intensity Pulsed Ultrasound Stimulation Based on Functional Cortico-Muscular Coupling

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Key result

LIPUS significantly improves neuromuscular functional preservation in cerebral ischemia models.

P<0.001
n=39

Why the study?

Low-intensity pulsed ultrasound stimulation (LIPUS) shows promise for ischemic stroke, but its underlying mechanisms lack in-depth investigation, especially regarding quantitative efficacy evaluation.

Does low-intensity pulsed ultrasound stimulation improve neuromuscular functional coupling in a mouse model of ischemic stroke?

Structured PICO

Does low-intensity pulsed ultrasound stimulation improve neuromuscular functional coupling in a mouse model of ischemic stroke?

P

Population

39 male C57BL/6 mice, of which 9 underwent bilateral carotid artery occlusion (BCAO) to model ischemic stroke.

I

Intervention

Low-intensity pulsed ultrasound stimulation (LIPUS) targeted to the primary motor cortex (fundamental frequency 500 kHz, pulse repetition frequency 1 kHz, duty cycle 50%, stimulation duration 300 ms, spatial peak pulse average intensity 0.95 W/cm²) for 30 cycles.

C

Comparator

Pre-stimulation state in the same BCAO mice (and comparison to normal control mice).

O

Outcome

Neuromuscular functional coupling quantified by a dynamic time warping (DTW) model-based percentage scoring system (DTW-100) using time- and frequency-domain analyses of local field potentials (LFPs) and electromyography (EMG).surrogate

Main Result

p-value: p=<0.001

Low-intensity pulsed ultrasound stimulation demonstrated neuroprotective effects in a mouse model of ischemic stroke by significantly improving cortico-muscular coupling, which was effectively quantified using a novel dynamic time warping algorithm.

Limitations

Small sample size
Advanced analytical approaches like deep learning were not yet incorporated
Use of global cerebral ischemia model (BCAO) may not fully reflect clinical heterogeneity compared to localized models
Lack of deep learning models for signal analysis
Use of a global ischemia model (BCAO) rather than localized ischemia models (e.g., MCAO) that better reflect clinical heterogeneity
Need for further algorithm optimization and control group-specific algorithms

Implication

Does not support clinical use of LIPUS in stroke; hypothesis-generating for neuromodulation in preclinical ischemia models.

Abstract

(1) Background: Ischemic stroke is a major global public-health concern with complex pathogenesis. Current treatment strategies face challenges. Low-intensity pulsed ultrasound stimulation (LIPUS), a non-invasive neuromodulation technology, shows promise in treating ischemic stroke, yet its underlying mechanisms lack in-depth investigation, especially in quantitative efficacy evaluation. (2) Methods: This study aimed to develop a neuromuscular functional coupling-based dynamic time warping (DTW) model to evaluate LIPUS's neuroprotective effects in a mouse model of ischemic stroke. A bilateral carotid artery occlusion (BCAO) model in mice was established, and LIPUS treatment was given. Time- and frequency-domain analyses of local field potentials (LFPs) and electromyography (EMG) were conducted, and outcomes were quantified using a percentage-based scoring system. (3) Results: The BCAO+LIPUS group scored significantly higher than the BCAO group. (4) Conclusions: This study demonstrated that LIPUS is neuroprotective in BCAO mice and that the DTW-100 assessment evaluation model can quantify the neuroprotective effects of LIPUS.

Discussion

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Figures from the paper

Published by Bioengineering · CC BY

Cite This Study

Jin et al. (2025) studied Ischemic Stroke (n=39). Low-intensity pulsed ultrasound stimulation (LIPUS) vs. Pre-stimulation BCAO state was evaluated on Neuromuscular functional coupling evaluated via dynamic time warping (DTW-100) scoring system (p=<0.001). LIPUS significantly improved DTW-100 scores in both time and frequency domains compared to baseline in BCAO mice (p<0.001), indicating neuroprotective functional preservation.

synapsesocial.com/papers/6a2385a38ac8ec529b08ce38 https://doi.org/10.3390/bioengineering12050541

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An Evaluation Model for Brain Ischemia Protection in Mice by Low-Intensity Pulsed Ultrasound Stimulation Based on Functional Cortico-Muscular Coupling

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Figures from the paper

Cite This Study

An Evaluation Model for Brain Ischemia Protection in Mice by Low-Intensity Pulsed Ultrasound Stimulation Based on Functional Cortico-Muscular Coupling

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Figures from the paper

Cite This Study

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