Atrial natriuretic factor augmented intracellular and extracellular cGMP in renal cells, and urinary cGMP correlated with ANF-induced natriuresis in awake rats.
Does urinary cGMP excretion reflect the renal activity of atrial natriuretic factor in vivo?
Urinary cGMP excretion correlates with ANF-induced natriuresis and may be a useful biological marker for the renal activity of ANF in vivo.
Current evidence suggests guanosine 3',5'-cyclic monophosphate (cGMP) serves as the second messenger for atrial natriuretic factor (ANF) in the kidney in vivo. We examined whether extracellular cGMP accumulation quantitatively reflected the concentration of cGMP within renal cells and whether urinary excretion of cGMP correlated with the physiological action of ANF. cGMP egression was examined in renal epithelial LLC-PK1 cells. ANF augmented intracellular cGMP concentration and extracellular cGMP appearance. Extracellular cGMP was an excellent function of the time-integrated intracellular cGMP concentration. In clearance studies in awake rats, urinary cGMP was primarily of renal cellular origin and correlated with the natriuresis induced by ANF in a time-dependent and concentration-dependent fashion. Urinary cGMP excretion may be useful as a biological marker for the renal activity of ANF in vivo.
Wong et al. (Thu,) reported a other. Atrial natriuretic factor (ANF) was evaluated on Extracellular cGMP accumulation and urinary excretion of cGMP. Atrial natriuretic factor augmented intracellular and extracellular cGMP in renal cells, and urinary cGMP correlated with ANF-induced natriuresis in awake rats.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: