Improvements in a method for localized antigen detection in tissue cells using fluorescein-labeled antibodies, indicating enhanced visualization techniques.
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
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Coons et al. (1950) studied this question.
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