Key result
Inhibition of contractile activity in cultured neonatal cardiac myocytes resulted in significantly greater perinuclear accumulation of reporter protein compared to contracting control cells (48.3% vs 29.1%, P<0.05).
Population
Cultured neonatal cardiac myocytes
Comparison
Alteration of spontaneous contractile activity vs Contracting control cells
Design
Preclinical
Follow-up
6 hours
Authors
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Should not change clinical management; leaves open whether contractile activity regulates mRNA localization in adult or in vivo myocardium.
Absolute Event Rate: 48.3% vs 29.1%
p-value: p=<0.05
Mechanical activity of neonatal cardiac myocytes regulates the intracellular localization of alpha-myosin heavy chain mRNA via its 3' untranslated region mediated by an initial block in translation.
Goldspink et al. (1997) studied this question. Contractile arrest (via verapamil or cycloheximide) vs. Contracting control cells was evaluated on Perinuclear accumulation of ss-galactosidase reporter protein (p=<0.05). Inhibition of contractile activity in cultured neonatal cardiac myocytes resulted in significantly greater perinuclear accumulation of reporter protein compared to contracting control cells (48.3% vs 29.1%, P<0.05).
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