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This study aims to understand how 1,25-(OH)2D3 affects the release and activation of latent TGF-beta by chondrocytes.

Latent transforming growth factor-beta is produced by chondrocytes and activated by extracellular matrix vesicles upon exposure to 1,25-(OH)2D3.

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This study aims to understand how 1,25-(OH)2D3 affects the release and activation of latent TGF-beta by chondrocytes.
Chondrocytes from resting and growth zones were cultured and treated with 1,25-(OH)2D3 and exogenous TGF-beta.
The release of latent TGF-beta into the culture medium was measured after treatment.
The ability of GC matrix vesicles to activate latent TGF-beta upon exposure to 1,25-(OH)2D3 was tested.
1,25-(OH)2D3 caused a dose-dependent inhibition of latent TGF-beta release, though mRNA levels for TGF-beta 1 remained unchanged.
Matrix vesicles activated latent TGF-beta 1 and TGF-beta 2 when preincubated with 1,25-(OH)2D3.
1,25-(OH)2D3 also inhibited the plasma membrane activation of latent TGF-beta.

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Randomized trial investigates how 1,25-(OH)2D3 regulates latent TGF-beta activation in chondrocytes, suggesting nongenomic mechanisms.

Boyan et al. (1994) studied this question.

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